PMID:12368266
| Citation |
Lloyd, GS, Niu, W, Tebbutt, J, Ebright, RH and Busby, SJ (2002) Requirement for two copies of RNA polymerase alpha subunit C-terminal domain for synergistic transcription activation at complex bacterial promoters. Genes Dev. 16:2557-65 |
|---|---|
| Abstract |
Transcription activation by the Escherichia coli cyclic AMP receptor protein (CRP) at different promoters has been studied using RNA polymerase holoenzyme derivatives containing two full-length alpha subunits, or containing one full-length alpha subunit and one truncated alpha subunit lacking the alpha C-terminal domain (alpha CTD). At a promoter having a single DNA site for CRP, activation requires only one full-length alpha subunit. Likewise, at a promoter having a single DNA site for CRP and one adjacent UP-element subsite (high-affinity DNA site for alpha CTD), activation requires only one full-length alpha subunit. In contrast, at promoters having two DNA sites for CRP, or one DNA site for CRP and two UP-element subsites, activation requires two full-length alpha subunits. We conclude that a single copy of alpha CTD is sufficient to interact with one CRP molecule and one adjacent UP-element subsite, but two copies of alpha CTD are required to interact with two CRP molecules or with one CRP molecule and two UP-element subsites. |
| Links |
PubMed PMC187446 Online version:10.1101/gad.237502 |
| Keywords |
Base Sequence; Binding Sites; Cyclic AMP Receptor Protein/metabolism; DNA, Bacterial; DNA-Directed RNA Polymerases/genetics; DNA-Directed RNA Polymerases/metabolism; Escherichia coli; Molecular Sequence Data; Promoter Regions, Genetic; Protein Structure, Tertiary; Transcriptional Activation |
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