PMID:12352954
| Citation |
Maris, AE, Sawaya, MR, Kaczor-Grzeskowiak, M, Jarvis, MR, Bearson, SM, Kopka, ML, Schröder, I, Gunsalus, RP and Dickerson, RE (2002) Dimerization allows DNA target site recognition by the NarL response regulator. Nat. Struct. Biol. 9:771-8 |
|---|---|
| Abstract |
Two-component signal transduction systems are modular phosphorelay regulatory pathways common in prokaryotes. In the co-crystal structure of the Escherichia coli NarL signal output domain bound to DNA, we observe how the NarL family of two-component response regulators can bind DNA. DNA recognition is accompanied by the formation of a new dimerization interface, which could occur only in the full-length protein via a large intramolecular domain rearrangement. The DNA is recognized by the concerted effects of solvation, van der Waals forces and inherent DNA deformability, rather than determined primarily by major groove hydrogen bonding. These subtle forces permit a small DNA-binding domain to perturb the DNA helix, leading to major DNA curvature and a transition from B- to A-form DNA at the binding site, where valine on the recognition helix interacts unexpectedly with the polar major groove floor. |
| Links |
PubMed Online version:10.1038/nsb845 |
| Keywords |
Bacterial Proteins/chemistry; Bacterial Proteins/metabolism; Cloning, Molecular; Crystallography, X-Ray; DNA/chemistry; DNA/metabolism; DNA-Binding Proteins/chemistry; DNA-Binding Proteins/metabolism; Dimerization; Escherichia coli; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Nucleic Acid Conformation; Promoter Regions, Genetic; Protein Binding; Protein Conformation; Protein Structure, Secondary; Recombinant Proteins/chemistry; Structure-Activity Relationship |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.