PMID:12270817
| Citation |
Rodriguez, C, Tompkin, J, Hazel, J and Foster, PL (2002) Induction of a DNA nickase in the presence of its target site stimulates adaptive mutation in Escherichia coli. J. Bacteriol. 184:5599-608 |
|---|---|
| Abstract |
Adaptive mutation to Lac(+) in Escherichia coli strain FC40 depends on recombination functions and is enhanced by the expression of conjugal functions. To test the hypothesis that the conjugal function that is important for adaptive mutation is the production of a single-strand nick at the conjugal origin, we supplied an exogenous nicking enzyme, the gene II protein (gIIp) of bacteriophage f1, and placed its target sequence near the lac allele. When both gIIp and its target site were present, adaptive mutation was stimulated three- to fourfold. Like normal adaptive mutations, gIIp-induced mutations were recA(+) and ruvC(+) dependent and were mainly single-base deletions in runs of iterated bases. In addition, gIIp with its target site could substitute for conjugal functions in adaptive mutation. These results support the hypothesis that nicking at the conjugal origin initiates the recombination that produces adaptive mutations in this strain of E. coli, and they suggest that nicking may be the only conjugal function required for adaptive mutation. |
| Links | |
| Keywords |
Adaptation, Physiological; Alleles; Conjugation, Genetic; Deoxyribonuclease I/biosynthesis; Enzyme Induction; Escherichia coli/enzymology; Escherichia coli/genetics; Lac Operon; Mutation; Recombination, Genetic; Viral Proteins/biosynthesis |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.