PMID:12244118
| Citation |
Eroglu, B and Powers-Lee, SG (2002) Unmasking a functional allosteric domain in an allosterically nonresponsive carbamoyl-phosphate synthetase. J. Biol. Chem. 277:45466-72 |
|---|---|
| Abstract |
Although carbamoyl-phosphate synthetases (CPSs) share sequence identity, multidomain structure, and reaction mechanism, they have varying physiological roles and allosteric effectors. Escherichia coli CPS (eCPS) provides CP for both arginine and pyrimidine nucleotide biosynthesis and is allosterically regulated by metabolites from both pathways, with inhibition by UMP and activation by IMP and ornithine. The arginine-specific CPS from Saccharomyces cerevisiae (sCPS), however, apparently responds to no allosteric effectors. We have designed and analyzed a chimeric CPS (chCPS, in which the C-terminal 136 residues of eCPS were replaced by the corresponding residues of sCPS) to define the structural basis for the allosteric nonresponsiveness of sCPS and thereby provide insight into the mechanism for allosteric selectivity and responsiveness in the other CPSs. Surprisingly, ornithine and UMP each had a significant effect on chCPS activity, and did so at concentrations that were similar to those effective for eCPS. We further found that sCPS bound both UMP and IMP and that chCPS bound IMP, although none of these interactions led to changes in enzymatic activity. These findings strongly suggest that the nonresponsive sCPS is not able to communicate occupancy of the allosteric site to the active site but does contain a latent allosteric interaction domain. |
| Links |
PubMed Online version:10.1074/jbc.M208185200 |
| Keywords |
Adenosine Diphosphate/metabolism; Adenosine Triphosphate/metabolism; Allosteric Regulation; Animals; Binding Sites; Carbon-Nitrogen Ligases/chemistry; Carbon-Nitrogen Ligases/genetics; Carbon-Nitrogen Ligases/isolation & purification; Carbon-Nitrogen Ligases/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Humans; Inosine Monophosphate/metabolism; Molecular Structure; Ornithine/metabolism; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/isolation & purification; Recombinant Fusion Proteins/metabolism; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins/chemistry; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/isolation & purification; Saccharomyces cerevisiae Proteins/metabolism; Uridine Monophosphate/metabolism; Uridine Triphosphate/metabolism |
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