PMID:12238334
| Citation |
Yamamura, E, Lee, EH, Kuzumaki, A, Uematsu, N, Nunoshiba, T, Kawata, M and Yamamoto, K (2002) Characterization of spontaneous mutation in the delta soxR and SoxS overproducing strains of Escherichia coli. J. Radiat. Res. 43:195-203 |
|---|---|
| Abstract |
To examine the role of the soxRS regulon in mutagenesis, we characterized the spontaneous mutations occurring in the endogenous tonB gene in the delta soxR strain and the SoxS overproducing strain of Escherichia coli. Neither the delta soxR strain nor the SoxS overproducing strain led to an enhancement or diminishment of the spontaneous mutation frequency. By DNA sequencing, we determined 50 spontaneous mutants from the delta soxR strains, and found that 36% were both base substitutions and IS insertions, 14% frameshifts and 10% deletions. Among the base substitutions, G:C-->T:A transversions and G:C-->A:T transitions predominated, followed by A:T-->T:A transversions. We determined 54 spontaneous mutants from the SoxS overproducing strains, and found that 37% were IS insertions, 31% base substitutions, 17% frameshifts, 9% deletions and 6% duplications. Among the base substitutions, G:C-->T:A transversions dominated, followed by A:T-->T:A transversions and G:C-->A:T transitions. These results were similar to those from the soxRS+ strains. Thus, it is suggested that the soxRS-regulated genes do not play a significant role in the defense against spontaneous mutagenesis. |
| Links | |
| Keywords |
Bacterial Proteins/biosynthesis; Base Sequence/genetics; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/biosynthesis; Mutation/genetics; Trans-Activators/biosynthesis; Transcription Factors/biosynthesis |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.