PMID:12234679

Citation	Rasmussen, TA and Nolan, JM (2002) G350 of Escherichia coli RNase P RNA contributes to Mg2+ binding near the active site of the enzyme. Gene 294:177-85
Abstract	G350 of Escherichia coli RNase P RNA is a highly conserved residue among all bacteria and lies near the known magnesium binding site for the RNase P ribozyme, helix P4. Mutations at G350 have a dramatic effect on substrate cleavage activity for both RNA alone and holoenzyme; the G350C mutation has the most severe phenotype. The G350C mutation also inhibits growth of cells that express the mutant RNA in vivo under conditions of magnesium starvation. The results suggest that G350 contributes to Mg(2+) binding at helix P4 of RNase P RNA.
Links	PubMed
Keywords	Base Sequence; Binding Sites/genetics; Binding, Competitive; Cell Division/drug effects; Cell Division/genetics; Edetic Acid/pharmacology; Endoribonucleases/genetics; Endoribonucleases/metabolism; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli Proteins; Genetic Complementation Test; Guanosine/genetics; Guanosine/metabolism; Holoenzymes/genetics; Holoenzymes/metabolism; Magnesium/metabolism; Molecular Sequence Data; Mutation; Nucleic Acid Conformation; RNA, Bacterial/chemistry; RNA, Bacterial/genetics; RNA, Bacterial/metabolism; RNA, Catalytic/genetics; RNA, Catalytic/metabolism; Ribonuclease P
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