PMID:12226668
| Citation |
Falnes, PØ, Johansen, RF and Seeberg, E (2002) AlkB-mediated oxidative demethylation reverses DNA damage in Escherichia coli. Nature 419:178-82 |
|---|---|
| Abstract |
The bacterial AlkB protein is known to be involved in cellular recovery from alkylation damage; however, the function of this protein remains unknown. AlkB homologues have been identified in several organisms, including humans, and a recent sequence alignment study has suggested that these proteins may belong to a superfamily of 2-oxoglutarate-dependent and iron-dependent oxygenases (2OG-Fe(ii)-oxygenases). Here we show that AlkB from Escherichia coli is indeed a 2-oxoglutarate-dependent and iron-dependent DNA repair enzyme that releases replication blocks in alkylated DNA by a mechanism involving oxidative demethylation of 1-methyladenine residues. This mechanism represents a new pathway for DNA repair and the third type of DNA damage reversal mechanism so far discovered. |
| Links |
PubMed Online version:10.1038/nature01048 |
| Keywords |
Adenine/analogs & derivatives; Adenine/metabolism; Alkylation; Base Sequence; Chromatography, High Pressure Liquid; DNA Damage; DNA Methylation; DNA Repair; DNA Replication; DNA, Bacterial/genetics; DNA, Bacterial/metabolism; DNA, Single-Stranded/genetics; DNA, Single-Stranded/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Formaldehyde/metabolism; Mixed Function Oxygenases/genetics; Mixed Function Oxygenases/metabolism; Oxidation-Reduction; Transformation, Bacterial |
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