PMID:12198182
| Citation |
Adam, P, Hecht, S, Eisenreich, W, Kaiser, J, Grawert, T, Arigoni, D, Bacher, A and Rohdich, F (2002) Biosynthesis of terpenes: studies on 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase. Proc. Natl. Acad. Sci. U.S.A. 99:12108-13 |
|---|---|
| Abstract |
Earlier in vivo studies showed the involvement of IspH protein in the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). We have demonstrated now that cell extract of an Escherichia coli strain engineered for hyperexpression of the ispH (lytB) gene catalyzes the in vitro conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into IPP and DMAPP. The reaction requires NADH, FAD, divalent cations (preferably Co2+), and probably one or more as-yet-unidentified proteins. The low intrinsic catalytic activities of wild-type E. coli cell extract and isolated chromoplasts of red pepper (Capsicum annuum) are enhanced by the addition of purified recombinant IspH protein. |
| Links |
PubMed PMC129406 Online version:10.1073/pnas.182412599 |
| Keywords |
Amino Acid Sequence; Animals; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Capsicum/enzymology; Capsicum/genetics; Cloning, Molecular; DNA, Bacterial/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins; Hemiterpenes; Molecular Sequence Data; Organophosphorus Compounds/metabolism; Oxidoreductases/genetics; Oxidoreductases/metabolism; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Sequence Homology, Amino Acid; Terpenes/chemistry; Terpenes/metabolism |
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