PMID:12182880
| Citation |
Min, KL, Viazovkina, E, Galarneau, A, Parniak, MA and Damha, MJ (2002) Oligonucleotides comprised of alternating 2'-deoxy-2'-fluoro-beta-D-arabinonucleosides and D-2'-deoxyribonucleosides (2'F-ANA/DNA 'altimers') induce efficient RNA cleavage mediated by RNase H. Bioorg. Med. Chem. Lett. 12:2651-4 |
|---|---|
| Abstract |
Chimeric oligonucleotides comprised of alternating residues of 2'-deoxy-2'-fluoro-D-arabinonucleic acid (2'F-ANA) and DNA were synthesized and evaluated for an important antisense property-the ability to elicit ribonuclease H (RNase H) degradation of complementary RNA. Experiments used both human RNase HII and Escherichia coli RNase HI. Mixed backbone oligomers comprising alternating three-nucleotide segments of 2'F-ANA and three-nucleotide segments of DNA were the most efficient at eliciting RNase H degradation of target RNA, and were significantly better than oligonucleotides entirely composed of DNA, suggesting that these mixed backbone oligonucleotides may be potent antisense agents. |
| Links | |
| Keywords |
Arabinonucleosides/chemistry; Hydrolysis; Oligonucleotides, Antisense/chemistry; Oligonucleotides, Antisense/pharmacology; RNA/metabolism; Ribonuclease H/metabolism |
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