PMID:12148987
| Citation |
Chin, JW, Santoro, SW, Martin, AB, King, DS, Wang, L and Schultz, PG (2002) Addition of p-azido-L-phenylalanine to the genetic code of Escherichia coli. J. Am. Chem. Soc. 124:9026-7 |
|---|---|
| Abstract |
We report the selection of a new orthogonal aminoacyl tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in response to the amber codon, TAG. The amino acid is incorporated in good yield with high fidelity and can be used to crosslink interacting proteins. |
| Links | |
| Keywords |
Azides/metabolism; Chloramphenicol O-Acetyltransferase/metabolism; Cross-Linking Reagents; DNA, Bacterial/genetics; DNA, Bacterial/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Methanococcus/enzymology; Myoglobin/biosynthesis; Phenylalanine/analogs & derivatives; Phenylalanine/metabolism; Spectrometry, Mass, Electrospray Ionization; Tyrosine-tRNA Ligase/metabolism |
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