PMID:12147516
| Citation |
Rajagopal, S, Sudarsan, N and Nickerson, KW (2002) Sodium dodecyl sulfate hypersensitivity of clpP and clpB mutants of Escherichia coli. Appl. Environ. Microbiol. 68:4117-21 |
|---|---|
| Abstract |
We studied the hypersensitivity of clpP and clpB mutants of Escherichia coli to sodium dodecyl sulfate (SDS). Both wild-type E. coli MC4100 and lon mutants grew in the presence of 10% SDS, whereas isogenic clpP and clpB single mutants could not grow above 0.5% SDS and clpA and clpX single mutants could not grow above 5.0% SDS. For wild-type E. coli, cellular ClpP levels as determined by Western immunoblot analysis increased ca. sixfold as the levels of added SDS increased from 0 to 2%. Capsular colanic acid, measured as uronic acid, increased ca. sixfold as the levels of added SDS increased from 2 to 10%. Based on these findings, 3 of the 19 previously identified SDS shock proteins (M. Adamowicz, P. M. Kelley, and K. W. Nickerson, J. Bacteriol. 173:229-233, 1991) are tentatively identified as ClpP, ClpX, and ClpB. |
| Links | |
| Keywords |
Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Culture Media; Drug Resistance, Bacterial; Endopeptidase Clp; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli Proteins; Heat-Shock Proteins/genetics; Heat-Shock Proteins/metabolism; Molecular Chaperones; Mutation; Serine Endopeptidases/genetics; Serine Endopeptidases/metabolism; Sodium Dodecyl Sulfate/pharmacology; Surface-Active Agents/pharmacology |
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