PMID:12144514
| Citation |
Lim, D and Jia, Z (2002) Heavy metal-mediated crystallization of Escherichia coli phytase and analysis of bridging interactions. Protein Pept. Lett. 9:359-65 |
|---|---|
| Abstract |
Escherichia coli phytase is a phosphatase that catalyzes the hydrolysis of phytic acid into inorganic phosphate and myo-inositol. Two crystal forms of this enzyme were obtained in the presence of heavy metals. Crystal forms I and II were obtained with the heavy atoms CdCl(2) and HgCl(2) and diffracted to 1.5 A and 2.25 A resolution, respectively. Hg(2+) and Cd(2+) both acted as molecular bridge(s), linking and stabilizing E. coli phytase in the unit cell, and played a crucial role in the crystallization of phytase by bridging neighbouring symmetry related molecules. |
| Links | |
| Keywords |
6-Phytase/chemistry; 6-Phytase/metabolism; Cadmium/chemistry; Crystallization; Escherichia coli/enzymology; Mercury/chemistry; Models, Molecular; Molecular Structure; Protein Conformation; Protein Structure, Tertiary |
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