PMID:12142434
| Citation |
Poteete, AR, Fenton, AC and Wang, HR (2002) Recombination-promoting activity of the bacteriophage lambda Rap protein in Escherichia coli K-12. J. Bacteriol. 184:4626-9 |
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| Abstract |
The rap gene of bacteriophage lambda was placed in the chromosome of an Escherichia coli K-12 strain in which the recBCD gene cluster had previously been replaced by the lambda red genes and in which the recG gene had been deleted. Recombination between linear double-stranded DNA molecules and the chromosome was tested in variants of the recGDelta red(+) rap(+) strain bearing mutations in genes known to affect recombination in other cellular pathways. The linear DNA was a 4-kb fragment containing the cat gene, with flanking lac sequences, released from an infecting phage chromosome by restriction enzyme cleavage in the cell. Replacement of wild-type lacZ with lacZ::cat was monitored by measuring the production of Lac-deficient chloramphenicol-resistant bacterial progeny. The results of these experiments indicated that the lambda rap gene could functionally substitute for the E. coli ruvC gene in Red-mediated recombination. |
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| Keywords |
Bacterial Proteins/genetics; Bacteriophage lambda/physiology; Chromosomes, Bacterial/genetics; Chromosomes, Bacterial/virology; Endodeoxyribonucleases/genetics; Escherichia coli/genetics; Escherichia coli/virology; Escherichia coli Proteins; Phenotype; Recombination, Genetic/physiology |
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