PMID:12138117

Citation	Veenendaal, AK, Van Der Does, C and Driessen, AJ (2002) The core of the bacterial translocase harbors a tilted transmembrane segment 3 of SecE. J. Biol. Chem. 277:36640-5
Abstract	The bacterial translocase mediates the translocation and membrane integration of proteins. The integral membrane proteins SecY and SecE are conserved core subunits of the translocase. Previous cysteine-scanning studies showed that the transmembrane segment (TMS) 3 of SecE contacts TMS 2 and 7 of SecY, and TMS 3 of another SecE. We now demonstrate that SecE also contacts TMS 10 of SecY. Combining all available cysteine-scanning mutagenesis data, a three-dimensional model has been built in which the positions of the helices that form the central core of the bacterial translocase are mapped. Remarkably, this model reveals that TMS 3 of SecE is strongly tilted relative to SecY.
Links	PubMed Online version:10.1074/jbc.M205713200
Keywords	Amino Acid Sequence; Cross-Linking Reagents/pharmacology; Cysteine/metabolism; Escherichia coli/enzymology; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Plasmids/metabolism; Protein Binding; Protein Conformation; Protein Structure, Tertiary; Protein Transport
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Add links to pages that link here (e.g. gene, product, method pages)

See Help:References for how to manage references in EcoliWiki.