PMID:12117918
| Citation |
Maeda, Y, Makino, M, Crick, DC, Mahapatra, S, Srisungnam, S, Takii, T, Kashiwabara, Y and Brennan, PJ (2002) Novel 33-kilodalton lipoprotein from Mycobacterium leprae. Infect. Immun. 70:4106-11 |
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| Abstract |
A novel Mycobacterium leprae lipoprotein LpK (accession no. ML0603) was identified from the genomic database. The 1,116-bp open reading frame encodes a 371-amino-acid precursor protein with an N-terminal signal sequence and a consensus motif for lipid conjugation. Expression of the protein, LpK, in Escherichia coli revealed a 33-kDa protein, and metabolic labeling experiments and globomycin treatment proved that the protein was lipidated. Fractionation of M. leprae demonstrated that this lipoprotein was a membrane protein of M. leprae. The purified lipoprotein was found to induce production of interleukin-12 in human peripheral blood monocytes. The studies imply that M. leprae LpK is involved in protective immunity against leprosy and may be a candidate for vaccine design. |
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| Keywords |
Amino Acid Sequence; Antigens, Bacterial/genetics; Antigens, Bacterial/immunology; Antigens, Bacterial/isolation & purification; Bacterial Proteins/genetics; Bacterial Proteins/immunology; Bacterial Proteins/isolation & purification; Cloning, Molecular; Escherichia coli; Gene Expression; Genes, Bacterial; Humans; Interleukin-12/biosynthesis; Lipoproteins/genetics; Lipoproteins/immunology; Lipoproteins/isolation & purification; Membrane Proteins/genetics; Membrane Proteins/immunology; Membrane Proteins/isolation & purification; Molecular Sequence Data; Molecular Weight; Monocytes/immunology; Mycobacterium leprae/genetics; Mycobacterium leprae/immunology; Mycobacterium leprae/isolation & purification; Protein Precursors/genetics; Protein Precursors/immunology; Protein Precursors/isolation & purification; Sequence Analysis, DNA |
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