PMID:12105207
| Citation |
Hayes, CS, Bose, B and Sauer, RT (2002) Proline residues at the C terminus of nascent chains induce SsrA tagging during translation termination. J. Biol. Chem. 277:33825-32 |
|---|---|
| Abstract |
The SsrA or tmRNA quality control system relieves ribosome stalling and directs the addition of a degradation tag to the C terminus of the nascent chain. In some instances, SsrA tagging of otherwise full-length proteins occurs when the ribosome pauses at stop codons during normal translation termination. Here, the identities of the C-terminal residues of the nascent chain are shown to play an important role in full-length protein tagging. Specifically, a subset of C-terminal Xaa-Pro sequences caused SsrA tagging of the full-length YbeL protein from Escherichia coli. This tagging increased when a less efficient stop codon was used, increased in cells lacking protein release factor-3, and decreased when protein release factor-1 was overexpressed. Incorporation of the analog azetidine-2-carboxylic acid in place of proline suppressed tagging, whereas incorporation of 3,4-dehydroproline increased SsrA tagging of full-length YbeL. These results suggest that the detailed chemical or conformational properties of the C-terminal residues of the nascent polypeptide can affect the rate of translation termination, thereby influencing ribosome pausing and SsrA tagging at stop codons. |
| Links |
PubMed Online version:10.1074/jbc.M205405200 |
| Keywords |
Codon, Terminator; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Proline/metabolism; Protein Biosynthesis; Protein Conformation; RNA, Bacterial/chemistry; RNA, Bacterial/metabolism; Ribosomes/metabolism |
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