PMID:12081641
| Citation |
Abo, T, Ueda, K, Sunohara, T, Ogawa, K and Aiba, H (2002) SsrA-mediated protein tagging in the presence of miscoding drugs and its physiological role in Escherichia coli. Genes Cells 7:629-38 |
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| Abstract |
We have shown recently that read-through of a normal stop codon by a suppressor tRNA in specific genes possessing a Rho-independent terminator leads to SsrA-mediated tagging of extended proteins in Escherichia coli cells. Miscoding antibiotics such as kanamycin and streptomycin reduce translational fidelity by binding to the 30S ribosomal subunit. The aim of the present study was to address how miscoding antibiotics affect the read-through of stop codons and SsrA-mediated protein tagging. |
| Links | |
| Keywords |
Aminoglycosides; Anti-Bacterial Agents/pharmacology; Carrier Proteins; Cyclic AMP Receptor Protein/genetics; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/metabolism; RNA-Binding Proteins/metabolism |
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