PMID:12076823

Citation	Schaller, A, Guo, M, Gisanrin, O and Zhang, Y (2002) Escherichia coli genes involved in resistance to pyrazinoic acid, the active component of the tuberculosis drug pyrazinamide. FEMS Microbiol. Lett. 211:265-70
Abstract	The natural resistance of Escherichia coli to pyrazinoic acid (POA), the active derivative of pyrazinamide, was investigated. The TolC mutant was found to be more susceptible to POA and other weak acids than the wild-type strain. Mutation in EmrB but not AcrB efflux protein slightly increased POA susceptibility. Two transposon mutants with increased susceptibility to POA were found to harbor mutations in acnA encoding aconitase-1 and ygiY encoding a putative two-component sensor protein. Complementation of the AcnA and YgiY mutants conferred resistance to POA, whereas the complemented TolC mutant became resistant to POA and other weak acids.
Links	PubMed
Keywords	Amidohydrolases/metabolism; Antitubercular Agents/pharmacology; Cloning, Molecular; Culture Media; DNA Transposable Elements; Drug Resistance, Microbial; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/metabolism; Genes, Bacterial; Mutation; Mycobacterium tuberculosis/drug effects; Nicotinamidase/metabolism; Pyrazinamide/analogs & derivatives; Pyrazinamide/metabolism; Pyrazinamide/pharmacology; Transformation, Bacterial
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Add links to pages that link here (e.g. gene, product, method pages)

See Help:References for how to manage references in EcoliWiki.