PMID:12065579

Citation	Geanacopoulos, M and Adhya, S (2002) Genetic analysis of GalR tetramerization in DNA looping during repressosome assembly. J. Biol. Chem. 277:33148-52
Abstract	The Gal repressosome is a nucleoprotein complex consisting of 2 GalR dimers, 1 HU, and 1 DNA loop, which represses the transcription of the gal operon. We have adopted a structure-based genetic approach to complement ongoing physical studies of the complex. Homology-based and subsequent alanine-scanning mutageneses suggest that five residues in the DNA-distal subdomain of GalR dimer are important for repressosome formation. A further analysis of these and intragenic suppressors of looping-defective GalR mutants as well as gain-of-function mutants that permit repressosome assembly in the absence of HU show that GalR dimers contact each other in the repressosome in a partially stacked configuration.
Links	PubMed Online version:10.1074/jbc.M202445200
Keywords	Alanine/chemistry; Alleles; DNA/chemistry; Dimerization; Escherichia coli/metabolism; Escherichia coli Proteins; Models, Genetic; Models, Molecular; Mutagenesis; Mutagenesis, Site-Directed; Mutation; Nucleic Acid Conformation; Plasmids/metabolism; Promoter Regions, Genetic; Protein Conformation; Repressor Proteins/chemistry; Transcription, Genetic
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