PMID:12052093

Citation	Khlebnikov, A and Keasling, JD Effect of lacY expression on homogeneity of induction from the P(tac) and P(trc) promoters by natural and synthetic inducers. Biotechnol. Prog. 18:672-4
Abstract	The role of the Escherichia coli lactose permease (LacY) in the homogeneous induction of the lactose-inducible promoters P(tac) and P(trc) by the natural inducer lactose and the synthetic inducer isopropyl-beta-D-thiogalactopyranoside (IPTG) was investigated. Lactose requires active transport by LacY, whereas IPTG can freely penetrate the cell wall. In E. coli strains lacking a functional LacY, IPTG is required for induction of P(tac) and P(trc). In E. coli strains carrying a functional LacY, induction of P(trc) and P(tac) with intermediate concentrations of lactose gave rise to two subpopulations, one fully induced and one uninduced, whereas a single, fully induced population resulted when high inducer concentrations were used. In contrast, induction with IPTG gave rise to a single population of cells at all inducer concentrations in both lacY and lacY(+) strains.
Links	PubMed Online version:10.1021/bp010141k
Keywords	Escherichia coli/genetics; Escherichia coli Proteins; Isopropyl Thiogalactoside; Membrane Transport Proteins/genetics; Monosaccharide Transport Proteins; Promoter Regions, Genetic; Symporters
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