PMID:12037319

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Citation

Krzywda, S, Brzozowski, AM, Karata, K, Ogura, T and Wilkinson, AJ (2002) Crystallization of the AAA domain of the ATP-dependent protease FtsH of Escherichia coli. Acta Crystallogr. D Biol. Crystallogr. 58:1066-7

Abstract

FtsH is a membrane-anchored ATP-dependent protease that degrades misfolded or misassembled membrane proteins as well as a subset of cytoplasmic regulatory proteins. It belongs to the family of AAA(+) ATPases with roles in diverse cellular processes. The ATPase domain of FtsH from Escherichia coli has been crystallized from ammonium sulfate solutions and crystals diffracting to 1.5 A resolution have been obtained.

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Keywords

ATP-Dependent Proteases; Bacterial Proteins/chemistry; Crystallization; Crystallography, X-Ray; Escherichia coli/enzymology; Escherichia coli Proteins; Heat-Shock Proteins/chemistry; Membrane Proteins/chemistry; Protein Conformation; Protein Structure, Tertiary; Serine Endopeptidases/chemistry

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