PMID:12034843
| Citation |
Tardif, KD and Horowitz, J (2002) Transfer RNA determinants for translational editing by Escherichia coli valyl-tRNA synthetase. Nucleic Acids Res. 30:2538-45 |
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| Abstract |
Valyl-tRNA synthetase (ValRS) has difficulty differentiating valine from structurally similar non-cognate amino acids, most prominently threonine. To minimize errors in aminoacylation and translation the enzyme catalyzes a proofreading (editing) reaction that is dependent on the presence of cognate tRNA(Val). Editing occurs at a site functionally distinct from the aminoacylation site of ValRS and previous results have shown that the 3'-terminus of tRNA(Val) is recognized differently at the two sites. Here, we extend these studies by comparing the contribution of aminoacylation identity determinants to productive recognition of tRNA(Val) at the aminoacylation and editing sites, and by probing tRNA(Val) for editing determinants that are distinct from those required for aminoacylation. Mutational analysis of Escherichia coli tRNA(Val) and identity switch experiments with non-cognate tRNAs reveal a direct relationship between the ability of a tRNA to be aminoacylated and its ability to stimulate the editing activity of ValRS. This suggests that at least a majority of editing by the enzyme entails prior charging of tRNA and that misacylated tRNA is a transient intermediate in the editing reaction. |
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| Keywords |
Acylation; Anticodon/genetics; Anticodon/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Mutation; Protein Biosynthesis/drug effects; Protein Biosynthesis/genetics; RNA Editing/drug effects; RNA, Bacterial/genetics; RNA, Bacterial/metabolism; RNA, Transfer/genetics; RNA, Transfer/metabolism; RNA, Transfer, Val/genetics; RNA, Transfer, Val/metabolism; Substrate Specificity; Threonine/metabolism; Threonine/pharmacology; Valine/metabolism; Valine-tRNA Ligase/genetics; Valine-tRNA Ligase/metabolism |
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