PMID:12005432
| Citation |
Ramón-Maiques, S, Marina, A, Gil-Ortiz, F, Fita, I and Rubio, V (2002) Structure of acetylglutamate kinase, a key enzyme for arginine biosynthesis and a prototype for the amino acid kinase enzyme family, during catalysis. Structure 10:329-42 |
|---|---|
| Abstract |
N-Acetyl-L-glutamate kinase (NAGK), a member of the amino acid kinase family, catalyzes the second and frequently controlling step of arginine synthesis. The Escherichia coli NAGK crystal structure to 1.5 A resolution reveals a 258-residue subunit homodimer nucleated by a central 16-stranded molecular open beta sheet sandwiched between alpha helices. In each subunit, AMPPNP, as an alphabetagamma-phosphate-Mg2+ complex, binds along the sheet C edge, and N-acetyl-L-glutamate binds near the dyadic axis with its gamma-COO- aligned at short distance from the gamma-phosphoryl, indicating associative phosphoryl transfer assisted by: (1) Mg2+ complexation; (2) the positive charges on Lys8, Lys217, and on two helix dipoles; and (3) by hydrogen bonding with the y-phosphate. The structural resemblance with carbamate kinase and the alignment of the sequences suggest that NAGK is a structural and functional prototype for the amino acid kinase family, which differs from other acylphosphate-making devices represented by phosphoglycerate kinase, acetate kinase, and biotin carboxylase. |
| Links | |
| Keywords |
Amino Acid Sequence; Arginine/biosynthesis; Binding Sites; Catalysis; Crystallography, X-Ray; Dimerization; Escherichia coli/enzymology; Molecular Sequence Data; Molecular Structure; Multigene Family; Phosphotransferases (Carboxyl Group Acceptor)/chemistry; Phosphotransferases (Carboxyl Group Acceptor)/genetics; Phosphotransferases (Carboxyl Group Acceptor)/metabolism; Protein Structure, Secondary; Protein Structure, Tertiary; Sequence Alignment |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.