PMID:11997517
| Citation |
Ludlam, WH, Taylor, MH, Tanner, KG, Denu, JM, Goodman, RH and Smolik, SM (2002) The acetyltransferase activity of CBP is required for wingless activation and H4 acetylation in Drosophila melanogaster. Mol. Cell. Biol. 22:3832-41 |
|---|---|
| Abstract |
CBP is a critical coactivator of transcription, but little is understood about the importance of its intrinsic acetyltransferase (AT) activity in gene activation in vivo. We show that the intrinsic AT function of CBP in Drosophila melanogaster (dCBP) is necessary to maintain a dCBP overexpression phenotype in the eye, for the in vivo activation of a specific target gene, wingless, and for the global acetylation of histone H4. These findings indicate that a point mutation which alters the intrinsic AT activity of CBP (only one of many CBP functions) has profound effects on CBP-induced gene activation in a physiologically intact transcription system. Furthermore, the effects of CBP AT activity are not limited to a few specific promoters, but rather CBT AT activity may play a role in regulating global histone acetylation throughout the developing organism. |
| Links | |
| Keywords |
Acetyl Coenzyme A/metabolism; Acetyltransferases/chemistry; Acetyltransferases/genetics; Acetyltransferases/metabolism; Amino Acid Motifs; Amino Acid Sequence; Animals; Binding Sites/genetics; CREB-Binding Protein; Drosophila Proteins; Drosophila melanogaster/embryology; Drosophila melanogaster/genetics; Drosophila melanogaster/metabolism; Gene Expression Regulation, Developmental; Genes, Insect; Histones/metabolism; Molecular Sequence Data; Nuclear Proteins/chemistry; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Phenotype; Point Mutation; Proto-Oncogene Proteins/genetics; Sequence Homology, Amino Acid; Trans-Activators/chemistry; Trans-Activators/genetics; Trans-Activators/metabolism; Transcriptional Activation; Wnt1 Protein |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.