PMID:11988530
| Citation |
Krin, E, Sismeiro, O, Danchin, A and Bertin, PN (2002) The regulation of Enzyme IIA(Glc) expression controls adenylate cyclase activity in Escherichia coli. Microbiology (Reading, Engl.) 148:1553-9 |
|---|---|
| Abstract |
During the last few years, several genes, such as pap, bgl and flhDC, have been shown to be coregulated by the histone-like nucleoid-structuring (H-NS) protein and the cyclic AMP-catabolite activator protein (cAMP/CAP) complex, suggesting an interaction between both systems in the control of some cellular functions. In this study, the possible effect of H-NS on the cAMP level was investigated. In a CAP-deficient strain, the presence of an hns mutation results in a strong reduction in the amount of cAMP, due to a decrease in adenylate cyclase activity. This is caused by the reduced expression of crr, which encodes the Enzyme IIA(Glc) of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS), from its specific P2 promoter. This leads to a twofold reduction in the global amount of Enzyme IIA(Glc), the adenylate cyclase activator, responsible for the decrease in adenylate cyclase activity observed in the hns crp strain. |
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| Keywords |
Adenylate Cyclase/biosynthesis; Adenylate Cyclase/genetics; Adenylate Cyclase/metabolism; Bacterial Proteins; Carrier Proteins; Cyclic AMP/metabolism; Cyclic AMP Receptor Protein/deficiency; Cyclic AMP Receptor Protein/genetics; DNA-Binding Proteins/deficiency; DNA-Binding Proteins/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Mutation/genetics; Operon/genetics; Phosphoenolpyruvate Sugar Phosphotransferase System/genetics; Phosphoenolpyruvate Sugar Phosphotransferase System/metabolism; Phosphorylation |
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