PMID:11985712
| Citation |
Yang, L, Lin, RT and Newman, EB (2002) Structure of the Lrp-regulated serA promoter of Escherichia coli K-12. Mol. Microbiol. 43:323-33 |
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| Abstract |
Expression of the Escherichia coli serA gene is activated in vivo by the product of the lrp gene, leucine-responsive regulatory protein (Lrp), an effect partially reversed by L-leucine. We show here that serA is transcribed from two promoters, P1 45 bp upstream of the translation start site, and P2 92 bp further upstream. Lrp binds to a long AT-rich sequence from -158 to -82 from the start of the coding region, i.e. upstream of P1 and overlapping P2. It activates transcription from P1 and represses expression from P2. A second regulator, cAMP/CRP, activates P2, an effect that is largely inhibited by Lrp, such that catabolite repressor protein (Crp) and Lrp are rival activators of serA transcription. |
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| Keywords |
Base Sequence; Binding Sites; Carbohydrate Dehydrogenases/genetics; Carrier Proteins; Chromosomes, Bacterial; Cyclic AMP/metabolism; Cyclic AMP Receptor Protein/metabolism; DNA Footprinting; DNA, Bacterial; DNA-Binding Proteins/biosynthesis; DNA-Binding Proteins/metabolism; Deoxyribonuclease I; Electrophoretic Mobility Shift Assay; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Lac Operon; Leucine-Responsive Regulatory Protein; Molecular Sequence Data; Mutagenesis, Site-Directed; Phosphoglycerate Dehydrogenase; Promoter Regions, Genetic; Recombinant Fusion Proteins/genetics; Transcription Factors |
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