PMID:11959127
| Citation |
Bagramyan, K, Mnatsakanyan, N, Poladian, A, Vassilian, A and Trchounian, A (2002) The roles of hydrogenases 3 and 4, and the F0F1-ATPase, in H2 production by Escherichia coli at alkaline and acidic pH. FEBS Lett. 516:172-8 |
|---|---|
| Abstract |
The hyc operon of Escherichia coli encodes the H2-evolving hydrogenase 3 (Hyd-3) complex that, in conjunction with formate dehydrogenase H (Fdh-H), constitutes a membrane-associated formate hydrogenlyase (FHL) catalyzing the disproportionation of formate to CO2 and H2 during fermentative growth at low pH. Recently, an operon (hyf) encoding a potential second H2-evolving hydrogenase (Hyd-4) was identified in E. coli. In this study the roles of the hyc- and hyf-encoded systems in formate-dependent H2 production and Fdh-H activity have been investigated. In cells grown on glucose under fermentative conditions at slightly acidic pH the production of H2 was mostly Hyd-3- and Fdh-H-dependent, and Fdh-H activity was also mainly Hyd-3-dependent. However, at slightly alkaline pH, H2 production was found to be largely Hyd-4, Fdh-H and F0F1-ATPase-dependent, and Fdh-H activity was partially dependent on Hyd-4 and F0F1-ATPase. These results suggest that, at slightly alkaline pH, H2 production and Fdh-H activity are dependent on both the F0F1-ATPase and a novel FHL, designated FHL-2, which is composed of Hyd-4 and Fdh-H, and is driven by a proton gradient established by the F0F1-ATPase. |
| Links | |
| Keywords |
Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/metabolism; Formate Dehydrogenases/genetics; Formate Dehydrogenases/metabolism; Genes, Bacterial; Hydrogen/metabolism; Hydrogen-Ion Concentration; Hydrogenase/genetics; Hydrogenase/metabolism; Multienzyme Complexes/genetics; Multienzyme Complexes/metabolism; Mutation; Proton-Translocating ATPases/genetics; Proton-Translocating ATPases/metabolism; Protoplasts/metabolism |
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