PMID:11959124

Citation	Zhuang, Z, Song, F, Martin, BM and Dunaway-Mariano, D (2002) The YbgC protein encoded by the ybgC gene of the tol-pal gene cluster of Haemophilus influenzae catalyzes acyl-coenzyme A thioester hydrolysis. FEBS Lett. 516:161-3
Abstract	This paper examines the catalytic function of the protein YbgC, encoded by the ybgC gene of the tol-pal gene cluster in Haemophilus influenzae. The YbgC protein, a homologue of the Pseudomonas sp. strain CBS3 4-hydroxybenzoyl-coenzyme A thioesterase, conserves the active site Asp residue associated with thioesterase activity. The H. influenzae ybgC gene was cloned and overexpressed in Escherichia coli. The recombinant protein was purified and tested for thioesterase activity towards acyl-CoA and acyl-N-acetylcysteamine thioesters. The YbgC protein catalyzes the hydrolysis of short chain aliphatic acyl-CoA thioesters, while the D18N YbgC mutant protein (prepared to serve as a control) does not.
Links	PubMed
Keywords	Acyl Coenzyme A/metabolism; Amino Acid Sequence; Cloning, Molecular; Genes, Bacterial; Haemophilus influenzae/enzymology; Haemophilus influenzae/genetics; Kinetics; Molecular Sequence Data; Multigene Family; Mutagenesis, Site-Directed; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Sequence Homology, Amino Acid; Substrate Specificity; Thiolester Hydrolases/genetics; Thiolester Hydrolases/metabolism
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