PMID:11952835
| Citation |
Sugaya, Y, Ihara, K, Masuda, Y, Ohtsubo, E and Maki, H (2002) Hyper-processive and slower DNA chain elongation catalysed by DNA polymerase III holoenzyme purified from the dnaE173 mutator mutant of Escherichia coli. Genes Cells 7:385-99 |
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| Abstract |
A strong mutator mutation, dnaE173, leads to a Glu612 --> Lys amino acid change in the alpha subunit of Escherichia coli DNA polymerase III (PolIII) holoenzyme and abolishes the proofreading function of the replicative enzyme without affecting the 3' --> 5' exonuclease activity of the epsilon subunit. The dnaE173 mutator is unique in its ability to induce sequence-substitution mutations, suggesting that an unknown function of the alpha subunit is hampered by the dnaE173 mutation. |
| Links | |
| Keywords |
Adenosine Triphosphate/metabolism; DNA Polymerase III/genetics; DNA Polymerase III/metabolism; DNA Replication/physiology; DNA, Bacterial/biosynthesis; DNA-Binding Proteins/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Holoenzymes/metabolism; Mutation; Potassium Chloride/metabolism; Silver Staining; Thymidine Monophosphate/metabolism |
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