PMID:11936098
| Citation |
Kubo, I (2002) Potentiometric phosphate-sensing system utilizing phosphate-binding protein. Anal Bioanal Chem 372:273-5 |
|---|---|
| Abstract |
A phosphate-detection system has been developed which uses phosphate-binding protein (PBP) from Escherichia coli. PBP was immobilized on a sheet of nitrocellulose membrane by cross-linking and the membrane potential of the immobilized PBP was measured. The response time of the system to phosphate was 5 min. The response was selective to phosphate among other anions. Under optimum conditions 0.1-1.5 mmol L(-1) phosphate can be determined with this system. |
| Links |
PubMed Online version:10.1007/s00216-001-1138-1 |
| Keywords |
Biosensing Techniques/methods; Calibration; Collodion/chemistry; Cross-Linking Reagents/chemistry; Escherichia coli/chemistry; Ion-Selective Electrodes; Membranes, Artificial; Phosphate-Binding Proteins/chemistry; Phosphates/analysis; Potentiometry |
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