PMID:11929550
| Citation |
Jain, C, Deana, A and Belasco, JG (2002) Consequences of RNase E scarcity in Escherichia coli. Mol. Microbiol. 43:1053-64 |
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| Abstract |
The endoribonuclease RNase E plays an important role in RNA processing and degradation in Escherichia coli. The construction of an E. coli strain in which the cellular concentration of RNase E can be precisely controlled has made it possible to examine and quantify the effect of RNase E scarcity on RNA decay, gene regulation and cell growth. These studies show that RNase E participates in a step in the degradation of its RNA substrates that is partially or fully rate-determining. Our data also indicate that E. coli growth requires a cellular RNase E concentration at least 10-20% of normal and that the feedback mechanism that limits overproduction of RNase E is also able to increase its synthesis when its concentration drops below normal. The magnitude of the in-crease in RNA longevity under conditions of RNase E scarcity may be limited by an alternative pathway for RNA degradation. Additional experiments show that RNase E is a stable protein in E. coli. No other E. coli gene product, when either mutated or cloned on a multicopy plasmid, seems to be capable of compensating for an inadequate supply of this essential protein. |
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| Keywords |
Cell Division; Endoribonucleases/genetics; Endoribonucleases/metabolism; Enzyme Stability; Escherichia coli/drug effects; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/growth & development; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Genes, Bacterial; Homeostasis; Isopropyl Thiogalactoside/pharmacology; RNA, Bacterial/metabolism; RNA, Messenger/metabolism; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism |
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