PMID:11919204

Citation	Diwa, AA and Belasco, JG (2002) Critical features of a conserved RNA stem-loop important for feedback regulation of RNase E synthesis. J. Biol. Chem. 277:20415-22
Abstract	RNase E is an important regulatory enzyme that governs the principal pathway for mRNA degradation in Escherichia coli. This endonuclease controls its own synthesis via a feedback mechanism in which the longevity of rne (RNase E) mRNA is modulated by a cis-acting sensory element that responds to changes in cellular RNase E activity. Previous research has shown that this element is an RNA stem-loop (hp2) within the 5'-untranslated region of the rne transcript. Here we report studies involving mutational analysis and phylogenetic comparison that have identified the features of rne hp2 important for its function. These comprise an internal loop flanked on one side by a 2-bp stem and a hairpin loop and on the other side by a longer stem whose sequence is inconsequential. A search of bacterial genome sequences suggests that regulation by an hp2-like element may be a unique evolutionary adaptation of the rne transcript that is not shared by other mRNAs.
Links	PubMed Online version:10.1074/jbc.M202313200
Keywords	Base Sequence; Endoribonucleases/biosynthesis; Escherichia coli/genetics; Evolution, Molecular; Molecular Sequence Data; Nucleic Acid Conformation; RNA, Bacterial/chemistry; RNA, Bacterial/genetics; RNA, Bacterial/metabolism
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