PMID:11918668

Citation	Colland, F, Fujita, N, Ishihama, A and Kolb, A (2002) The interaction between sigmaS, the stationary phase sigma factor, and the core enzyme of Escherichia coli RNA polymerase. Genes Cells 7:233-47
Abstract	The RNA polymerase holoenzyme of Escherichia coli is composed of a core enzyme (subunit structure alpha2betabeta') associated with one of the sigma subunits, required for promoter recognition. Different sigma factors compete for core binding. Among the seven sigma factors present in E. coli, sigma70 controls gene transcription during the exponential phase, whereas sigmaS regulates the transcription of genes in the stationary phase or in response to different stresses. Using labelled sigmaS and sigma70, we compared the affinities of both sigma factors for core binding and investigated the structural changes in the different subunits involved in the formation of the holoenzymes.
Links	PubMed
Keywords	Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Binding Sites; Cysteine/metabolism; DNA-Directed RNA Polymerases/genetics; DNA-Directed RNA Polymerases/metabolism; Edetic Acid/analogs & derivatives; Electrophoretic Mobility Shift Assay; Escherichia coli/enzymology; Escherichia coli/genetics; Mutagenesis, Site-Directed; Protein Binding; Protein Footprinting; Sequence Analysis, Protein; Sigma Factor/genetics; Sigma Factor/metabolism
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