PMID:11894914
| Citation |
Marchal, C and Hofnung, M (1983) Negative dominance in gene lamB: random assembly of secreted subunits issued from different polysomes. EMBO J. 2:81-6 |
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| Abstract |
lamB is the structural gene for the lambda receptor, an oligomeric outer membrane protein from Escherichia coli K12 involved in phage lambda adsorption. We show that, under certain conditions, in a strain diploid for gene lamB, all the missense lamB mutations conferring lambda resistance that we have tested are dominant with respect to wild-type. We propose a model which allows a quantitative interpretation of the data. It is based on negative complementation at the level of oligomerisation. Wild-type and mutant subunits would assemble at random forming homo- and hetero-oligomers. Only wild-type homo-oligomers would be efficient for phage inactivation. For some classes of missense mutations the hetero-oligomers would have the capacity to bind, but not to inactivate the phage. The model confirms that active lambda receptor is a trimer and implies that for this secreted protein there is no preferential assembly of subunits originating from the same polysome. |
| Links | |
| Keywords |
Bacterial Outer Membrane Proteins/genetics; Escherichia coli/genetics; Genes, Bacterial; Polyribosomes; Porins; Receptors, Virus/genetics |
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