PMID:11894902
| Citation |
Hall, MN, Gabay, J and Schwartz, M (1983) Evidence for a coupling of synthesis and export of an outer membrane protein in Escherichia coli. EMBO J. 2:15-9 |
|---|---|
| Abstract |
We describe a lesion, lamB701-708, affecting the hydrophilic portion of the lambda receptor signal sequence. The C to A transversion of the sixth codon of the signal sequence changes a positively charged arginine to a neutral serine. The phenotype conferred by this alteration is unique among previously described signal sequence mutations. The results suggest an essential role for the charged amino acids of the hydrophilic segment in the initial interaction between a nascent secreted protein and a membrane export site. The results further suggest that synthesis of lambda receptor is coupled to its export. |
| Links | |
| Keywords |
Artificial Gene Fusion; Bacterial Outer Membrane Proteins/biosynthesis; Bacterial Outer Membrane Proteins/genetics; Bacterial Outer Membrane Proteins/metabolism; Bacteriophage lambda/metabolism; Biological Transport; Escherichia coli/genetics; Escherichia coli/metabolism; Genes, Bacterial; Lac Operon; Mutagenesis; Peptide Chain Initiation, Translational; Porins; Receptors, Virus/biosynthesis; Receptors, Virus/genetics; Receptors, Virus/metabolism; Transcription, Genetic |
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