PMID:11867724
| Citation |
Drew, D, Sjöstrand, D, Nilsson, J, Urbig, T, Chin, CN, de Gier, JW and von Heijne, G (2002) Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis. Proc. Natl. Acad. Sci. U.S.A. 99:2690-5 |
|---|---|
| Abstract |
We present an approach that allows rapid determination of the topology of Escherichia coli inner-membrane proteins by a combination of topology prediction and limited fusion-protein analysis. We derive new topology models for 12 inner-membrane proteins: MarC, PstA, TatC, YaeL, YcbM, YddQ, YdgE, YedZ, YgjV, YiaB, YigG, and YnfA. We estimate that our approach should make it possible to arrive at highly reliable topology models for roughly 10% of the approximately 800 inner-membrane proteins thought to exist in E. coli. |
| Links |
PubMed PMC122409 Online version:10.1073/pnas.052018199 |
| Keywords |
Alkaline Phosphatase; Bacterial Proteins/metabolism; Cyclin-Dependent Kinases/genetics; Cyclin-Dependent Kinases/metabolism; Escherichia coli/metabolism; Escherichia coli Proteins; Genes, Reporter; Green Fluorescent Proteins; Luminescent Proteins/genetics; Luminescent Proteins/metabolism; Membrane Proteins/metabolism; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Time Factors |
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