PMID:11826982

Citation	Ando, T, Tanaka, T, Hori, Y, Sakai, E and Kikuchi, Y (2001) Human tyrosine tRNA is also internally cleavable by E. coli ribonuclease P RNA ribozyme in vitro. Biosci. Biotechnol. Biochem. 65:2798-801
Abstract	Transfer RNA is an essential molecule for biological system, and each tRNA molecule commonly has a cloverleaf structure. Previously, we experimentally showed that some Drosophila tRNA (tRNA(Ala), tRNA(His), and tRNA(iMet)) molecules fit to form another, non-cloverleaf, structure in which the 3'-half of the tRNA molecules forms an alternative hairpin, and that the tRNA molecules are internally cleaved by the catalytic RNA of bacterial ribonuclease P (RNase P). Until now, the hyperprocessing reaction of tRNA has only been reported with Drosophila tRNAs. This time, we applied the hyperprocessing reaction to one of human tRNAs, human tyrosine tRNA, and we showed that this tRNA was also hyperprocessed by E. coli RNase P RNA. This tRNA is the first example for hyperprocessed non-Drosophila tRNAs. The results suggest that the hyperprocessing reaction can be a useful tool detect destablized tRNA molecules from any species.
Links	PubMed
Keywords	Base Sequence; Electrophoresis, Polyacrylamide Gel; Endoribonucleases/metabolism; Escherichia coli/enzymology; Escherichia coli Proteins; Humans; Hydrolysis; Molecular Sequence Data; Nucleic Acid Conformation; RNA, Catalytic/metabolism; RNA, Transfer, Tyr/chemistry; RNA, Transfer, Tyr/metabolism; Ribonuclease P
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Add links to pages that link here (e.g. gene, product, method pages)

See Help:References for how to manage references in EcoliWiki.