PMID:11821429

Citation	Urbanus, ML, Fröderberg, L, Drew, D, Björk, P, de Gier, JW, Brunner, J, Oudega, B and Luirink, J (2002) Targeting, insertion, and localization of Escherichia coli YidC. J. Biol. Chem. 277:12718-23
Abstract	YidC was recently shown to play an important role in the assembly of inner membrane proteins (IMPs) both in conjunction with and separate from the Sec-translocon. Little is known about the biogenesis and structural and functional properties of YidC, itself a polytopic IMP. Here we analyze the targeting and membrane integration of YidC using in vivo and in vitro approaches. The combined data indicate that YidC is targeted by the signal recognition particle and inserts at the SecAYEG-YidC translocon early during biogenesis, unlike its mitochondrial homologue Oxa1p. In addition, YidC is shown to be relatively abundant compared with other components involved in IMP assembly and is predominantly localized at the poles of the cell.
Links	PubMed Online version:10.1074/jbc.M200311200
Keywords	Bacterial Proteins/metabolism; Escherichia coli/metabolism; Escherichia coli Proteins/metabolism; Membrane Transport Proteins; Protein Binding
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