PMID:11821048
| Citation |
van Dalen, A, van der Laan, M, Driessen, AJ, Killian, JA and de Kruijff, B (2002) Components required for membrane assembly of newly synthesized K+ channel KcsA. FEBS Lett. 511:51-8 |
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| Abstract |
An Escherichia coli in vitro transcription-translation system was used to study the components involved in the biogenesis of the homotetrameric potassium channel KcsA. We show that a functional signal recognition particle pathway is essential for tetramer formation, probably to direct correct monomer insertion in the membrane. In the absence of YidC or at reduced SecYEG levels, KcsA assembly occurs with lower efficiency. Strikingly, the highest efficiency of tetramerization was observed when transcription-translation was carried out in the presence of pure lipid vesicles, demonstrating that a phospholipid bilayer is the minimal membrane requirement to form the KcsA tetramer. It is concluded that SecYEG and YidC are not required for the formation of tetrameric KcsA in vitro. |
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| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Deletion; Lipid Bilayers/chemistry; Lipid Bilayers/metabolism; Liposomes/chemistry; Liposomes/metabolism; Membrane Proteins/biosynthesis; Membrane Proteins/chemistry; Membrane Proteins/metabolism; Membrane Transport Proteins; Potassium Channels/biosynthesis; Potassium Channels/chemistry; Potassium Channels/genetics; Potassium Channels/metabolism; Protein Biosynthesis; Protein Processing, Post-Translational; Protein Structure, Quaternary; Protein Subunits; Proteolipids/chemistry; Proteolipids/metabolism; Signal Transduction; Transcription, Genetic |
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