PMID:11818558
| Citation |
Rohdich, F, Hecht, S, Gärtner, K, Adam, P, Krieger, C, Amslinger, S, Arigoni, D, Bacher, A and Eisenreich, W (2002) Studies on the nonmevalonate terpene biosynthetic pathway: metabolic role of IspH (LytB) protein. Proc. Natl. Acad. Sci. U.S.A. 99:1158-63 |
|---|---|
| Abstract |
Isopentenyl diphosphate and dimethylallyl diphosphate serve as the universal precursors for the biosynthesis of terpenes. Although their biosynthesis by means of mevalonate has been studied in detail, a second biosynthetic pathway for their formation by means of 1-deoxy-D-xylulose 5-phosphate has been discovered only recently in plants and certain eubacteria. Earlier in vivo experiments with recombinant Escherichia coli strains showed that exogenous 1-deoxy-D-xylulose can be converted into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate by the consecutive action of enzymes specified by the xylB and ispCDEFG genes. This article describes the transformation of exogenous [U-(13)C(5)]1-deoxy-D-xylulose into a 5:1 mixture of [U-(13)C(5)]isopentenyl diphosphate and [U-(13)C(5)]dimethylallyl diphosphate by an E. coli strain engineered for the expression of the ispH (lytB) gene in addition to recombinant xylB and ispCDEFG genes. |
| Links |
PubMed PMC122160 Online version:10.1073/pnas.032658999 |
| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Enterobacteriaceae/genetics; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Magnetic Resonance Spectroscopy; Mevalonic Acid/metabolism; Molecular Sequence Data; Mycobacterium tuberculosis/genetics; Oligodeoxyribonucleotides/chemistry; Operon; Oxidoreductases; Promoter Regions, Genetic; Recombinant Proteins/metabolism; Terpenes/metabolism |
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