PMID:11815290
| Citation |
Kuo, WN, Kocis, JM and Webb, JK (2002) Protein denitration/modification by Escherichia coli nitrate reductase and mammalian cytochrome P-450 reductase. Front. Biosci. 7:a9-14 |
|---|---|
| Abstract |
The incubation of peroxynitrite (PN)-pretreated histone III-S (NH) with Escherichia coli nitrate reductase (cytochrome, NADPH/GSH-independent) and that of NADPH-treated NH (NHNADPH) with liver cytochrome P-450 reductase (NADPH-dependent) resulted in decreased 3-nitrotyrosine immunoreactivity found in Western blot analysis. Additionally, increased nitrate was noted as an end product of these reactions. These findings imply that varied enzymatic denitration/modification of NO/PN-reacted protein, either with or without a reductant, may be important in regulating related signal transduction cascade(s) and relieving oxidative stress. |
| Links | |
| Keywords |
Animals; Escherichia coli/enzymology; Histones/metabolism; Models, Chemical; NADP/chemistry; NADPH-Ferrihemoprotein Reductase/metabolism; Nitrate Reductase; Nitrate Reductases/metabolism; Nitrates/metabolism; Peroxynitrous Acid/chemistry; Tyrosine/analogs & derivatives; Tyrosine/analysis |
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