PMID:11811537
| Citation |
Benov, L and Al-Ibraheem, J (2001) Glycerol metabolism in superoxide dismutase-deficient Escherichia coli. Free Radic. Res. 35:867-72 |
|---|---|
| Abstract |
Escherichia coli, which lacks cytoplasmic superoxide dismutases, exhibits various phenotypic deficits if grown aerobically. Here we report that sodAsodB E. coli cannot use glycerol under aerobic conditions. The reason is low activity of glycerol kinase (GK), the rate-limiting enzyme in glycerol metabolism. Superoxide does not inactivate GK, but makes it susceptible to inactivation by a heat-labile factor present in the cell-free extracts. This factor seems to be part of a proteolytic system, which recognizes and degrades oxidatively modified proteins. |
| Links | |
| Keywords |
Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/metabolism; Glycerol/metabolism; Glycerol Kinase/metabolism; Paraquat/pharmacology; Superoxide Dismutase/deficiency; Superoxide Dismutase/genetics; Superoxide Dismutase/metabolism; Time Factors; Xanthine Oxidase/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.