PMID:11807083

Citation	Xu, W, Pagel, FT and Murgola, EJ (2002) Mutations in the GTPase center of Escherichia coli 23S rRNA indicate release factor 2-interactive sites. J. Bacteriol. 184:1200-3
Abstract	Mutations in the GTPase center of Escherichia coli 23S rRNA were characterized in vivo as UGA-specific nonsense suppressors. Some site-directed mutations did not exhibit suppressor activity and were interspersed among suppressor mutations. Our results demonstrate the involvement of the two adjacent loops of this conserved rRNA structure in UGA-dependent translation termination and, taken with previous in vitro analyses and with consideration of the crystal structure of the GTPase center RNA, indicate that nucleotides 1067, 1093, 1094, and 1095 are sites of interaction with release factor 2.
Links	PubMed PMC134791
Keywords	Binding Sites; Escherichia coli/genetics; Escherichia coli/growth & development; GTP Phosphohydrolases/metabolism; Gene Expression Regulation, Bacterial; Mutagenesis, Site-Directed; Nucleic Acid Conformation; Peptide Termination Factors/metabolism; RNA, Bacterial/chemistry; RNA, Ribosomal, 23S/chemistry; Ribosomal Proteins/metabolism; rRNA Operon
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Add links to pages that link here (e.g. gene, product, method pages)

See Help:References for how to manage references in EcoliWiki.