PMID:11804608
| Citation |
Timms, AR and Bridges, BA (2002) DNA polymerase V-dependent mutator activity in an SOS-induced Escherichia coli strain with a temperature-sensitive DNA polymerase III. Mutat. Res. 499:97-101 |
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| Abstract |
The temperature-sensitive DNA polymerase III (Pol III) encoded by the dnaE486 allele confers a spontaneous mutator activity in SOS-induced bacteria that is largely dependent upon DNA polymerase V (Pol V), encoded by umuD, C. This mutator activity is influenced by the defective proof-reading sub-unit of Pol III encoded by the dnaQ905 (mutD5) allele arguing that Pol V is most likely fixing mutations arising from mismatched primer termini produced by Pol III(486). The size of the dnaQ effect is, however, modest leaving open the possibility that Pol V may be responsible for some of the mutator effect by engaging in bursts of processive activity. |
| Links | |
| Keywords |
DNA Polymerase III/genetics; DNA Polymerase III/metabolism; DNA-Directed DNA Polymerase/physiology; Escherichia coli/physiology; Escherichia coli Proteins/genetics; Exodeoxyribonuclease V; Exodeoxyribonucleases/genetics; Mutation; SOS Response (Genetics); Temperature |
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