PMID:11790763

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Citation

Onogi, T, Ohsumi, K, Katayama, T and Hiraga, S (2002) Replication-dependent recruitment of the beta-subunit of DNA polymerase III from cytosolic spaces to replication forks in Escherichia coli. J. Bacteriol. 184:867-70

Abstract

The beta-subunit of DNA polymerase III is located as one or two condensed clusters within the nucleoid-occupied space in exponentially growing cells of Escherichia coli. When chromosome replication is terminated after incubation at nonpermissive temperature in a temperature-sensitive dnaC mutant, the beta-subunit is located in the cytosolic spaces of the cell poles.

Links

PubMed PMC139520

Keywords

Bacterial Outer Membrane Proteins; Cell Compartmentation; Chromosomes, Bacterial; Cytosol/enzymology; DNA Polymerase III/isolation & purification; DNA Replication; DNA, Bacterial/biosynthesis; DNA-Binding Proteins; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins; Protein Subunits; Transcription Factors/isolation & purification

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