PMID:11751638
| Citation |
Sukhodolets, MV, Cabrera, JE, Zhi, H and Jin, DJ (2001) RapA, a bacterial homolog of SWI2/SNF2, stimulates RNA polymerase recycling in transcription. Genes Dev. 15:3330-41 |
|---|---|
| Abstract |
We report that RapA, an Escherichia coli RNA polymerase (RNAP)-associated homolog of SWI2/SNF2, is capable of dramatic activation of RNA synthesis. The RapA-mediated transcriptional activation in vitro depends on supercoiled DNA and high salt concentrations, a condition that is likely to render the DNA superhelix tightly compacted. Moreover, RapA activates transcription by stimulating RNAP recycling. Mutational analyses indicate that the ATPase activity of RapA is essential for its function as a transcriptional activator, and a rapA null mutant exhibits a growth defect on nutrient plates containing high salt concentrations in vivo. Thus, RapA acts as a general transcription factor and an integral component of the transcription machinery. The mode of action of RapA in remodeling posttranscription or posttermination complexes is discussed. |
| Links |
PubMed PMC312849 Online version:10.1101/gad.936701 |
| Keywords |
Adenosine Triphosphatases/metabolism; Bacterial Proteins/isolation & purification; Bacterial Proteins/metabolism; Chromatin/chemistry; DNA Helicases; DNA Primers/chemistry; DNA-Binding Proteins/metabolism; DNA-Directed RNA Polymerases/genetics; DNA-Directed RNA Polymerases/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression; Nuclear Proteins; Nucleic Acid Conformation; Phosphoprotein Phosphatases/isolation & purification; Phosphoprotein Phosphatases/metabolism; Plasmids; RNA/metabolism; Transcription Factors/chemistry; Transcription Factors/isolation & purification; Transcription Factors/metabolism |
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