PMID:11751578
| Citation |
Pereira, PJ, Vega, MC, González-Rey, E, Fernández-Carazo, R, Macedo-Ribeiro, S, Gomis-Rüth, FX, González, A and Coll, M (2002) Trypanosoma cruzi macrophage infectivity potentiator has a rotamase core and a highly exposed alpha-helix. EMBO Rep. 3:88-94 |
|---|---|
| Abstract |
The macrophage infectivity potentiator protein from Trypanosoma cruzi (TcMIP) is a major virulence factor secreted by the etiological agent of Chagas' disease. It is functionally involved in host cell invasion. We have determined the three-dimensional crystal structure of TcMIP at 1.7 A resolution. The monomeric protein displays a peptidyl-prolyl cis-trans isomerase (PPIase) core, encompassing the characteristic rotamase hydrophobic active site, thus explaining the strong inhibition of TcMIP by the immunosuppressant FK506 and related drugs. In TcMIP, the twisted beta-sheet of the core is extended by an extra beta-strand, preceded by a long, exposed N-terminal alpha-helix, which might be a target recognition element. An invasion assay shows that the MIP protein from Legionella pneumophila (LpMIP), which has an equivalent N-terminal alpha-helix, can substitute for TcMIP. An additional exposed alpha-helix, this one unique to TcMIP, is located in the C-terminus of the protein. The high-resolution structure reported here opens the possibility for the design of new inhibitory drugs that might be useful for the clinical treatment of American trypanosomiasis. |
| Links |
PubMed PMC1083928 Online version:10.1093/embo-reports/kvf009 |
| Keywords |
Amino Acid Sequence; Animals; Bacterial Proteins; Binding Sites; Humans; Immunophilins/chemistry; Macrophages; Membrane Proteins/chemistry; Models, Molecular; Molecular Sequence Data; Peptidylprolyl Isomerase/chemistry; Protein Structure, Secondary; Protozoan Proteins/chemistry; Protozoan Proteins/physiology; Sequence Homology; Sequence Homology, Amino Acid; Tacrolimus Binding Proteins/chemistry; Trypanosoma cruzi/chemistry; Trypanosoma cruzi/enzymology; Trypanosoma cruzi/physiology |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.