PMID:11744738

Citation	Belogrudov, GI and Hatefi, Y (2002) Factor B and the mitochondrial ATP synthase complex. J. Biol. Chem. 277:6097-103
Abstract	Factor B is a subunit of the mammalian ATP synthase complex, whose existence has been controversial. This paper describes the molecular and functional properties of a recombinant human factor B, which when added to bovine submitochondrial particles depleted of their factor B restores the energy coupling activity of the ATP synthase complexes. The mature human factor B has 175 amino acids and a molecular mass of 20,341 Da. The preparation is water-soluble, monomeric, and is inactivated by monothiol- and especially dithiol-modifying reagents, probably reacting at its cysteine residues Cys-92 and Cys-94. A likely factor B gene composed of 5 exons has been identified on chromosome 14q21.3, and the functional role of factor B in the mammalian ATP synthase complex has been discussed.
Links	PubMed Online version:10.1074/jbc.M111256200
Keywords	Amino Acid Sequence; Animals; Base Sequence; Binding Sites; Cattle; Chromosome Mapping; Cloning, Molecular; Cysteine; Electron Transport/drug effects; Exons; Humans; Kinetics; Mitochondria, Heart/enzymology; Mitochondrial Proton-Translocating ATPases/chemistry; Mitochondrial Proton-Translocating ATPases/genetics; Mitochondrial Proton-Translocating ATPases/metabolism; Molecular Sequence Data; Molecular Weight; Oligomycins/pharmacology; Protein Subunits; Recombinant Proteins/chemistry; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Sulfhydryl Compounds/pharmacology
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