PMID:11742093

Citation	Liu, Y, Bondarenko, V, Ninfa, A and Studitsky, VM (2001) DNA supercoiling allows enhancer action over a large distance. Proc. Natl. Acad. Sci. U.S.A. 98:14883-8
Abstract	Enhancers are regulatory DNA elements that can activate their genomic targets over a large distance. The mechanism of enhancer action over large distance is unknown. Activation of the glnAp2 promoter by NtrC-dependent enhancer in Escherichia coli was analyzed by using a purified system supporting multiple-round transcription in vitro. The data suggest that DNA supercoiling is an essential requirement for enhancer action over a large distance (2,500 bp) but not over a short distance (110 bp). DNA supercoiling facilitates functional enhancer-promoter communication over a large distance, probably by bringing the enhancer and promoter into close proximity.
Links	PubMed PMC64953 Online version:10.1073/pnas.261477898
Keywords	Base Sequence; DNA Primers; DNA, Superhelical/physiology; Enhancer Elements, Genetic; Escherichia coli/genetics; Gene Expression Regulation, Bacterial; Promoter Regions, Genetic; Transcription, Genetic
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