PMID:11742080
| Citation |
Schwartz, CJ, Giel, JL, Patschkowski, T, Luther, C, Ruzicka, FJ, Beinert, H and Kiley, PJ (2001) IscR, an Fe-S cluster-containing transcription factor, represses expression of Escherichia coli genes encoding Fe-S cluster assembly proteins. Proc. Natl. Acad. Sci. U.S.A. 98:14895-900 |
|---|---|
| Abstract |
IscR (iron-sulfur cluster regulator) is encoded by an ORF located immediately upstream of genes coding for the Escherichia coli Fe-S cluster assembly proteins, IscS, IscU, and IscA. IscR shares amino acid similarity with MarA, a member of the MarA/SoxS/Rob family of transcription factors. In this study, we found that IscR functions as a repressor of the iscRSUA operon, because strains deleted for iscR have increased expression of this operon. In addition, in vitro transcription reactions established a direct role for IscR in repression of the iscR promoter. Analysis of IscR by electron paramagnetic resonance showed that the anaerobically isolated protein contains a [2Fe-2S](1+) cluster. The Fe-S cluster appears to be important for IscR function, because repression of iscR expression is significantly reduced in strains containing null mutations of the Fe-S cluster assembly genes iscS or hscA. The finding that IscR activity is decreased in strain backgrounds in which Fe-S cluster assembly is impaired suggests that this protein may be part of a novel autoregulatory mechanism that senses the Fe-S cluster assembly status of cells. |
| Links |
PubMed PMC64955 Online version:10.1073/pnas.251550898 |
| Keywords |
Amino Acid Sequence; DNA, Bacterial; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation, Bacterial/physiology; Genes, Bacterial; Iron-Sulfur Proteins/genetics; Molecular Sequence Data; Promoter Regions, Genetic; Repressor Proteins/chemistry; Repressor Proteins/genetics; Repressor Proteins/physiology; Sequence Homology, Amino Acid; Transcription Factors/chemistry; Transcription Factors/genetics; Transcription Factors/physiology |
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